Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody

EIF4E antibody

Boster Bio Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody catalog # P00135. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P00135
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody

View all EIF4E Antibodies

SKU/Catalog Number

P00135

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody catalog # P00135. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00135)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DBD-5

Immunogen

A synthesized peptide derived from human Phospho-eIF4E (S209)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00135 is reactive to EIF4E in Human, Mouse, Rat

Applications

P00135 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:1000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For EIF4E (Source: Uniprot.Org, NCBI)

Uniprot ID

P06730

Gene ID

1977

Gene Name

EIF4E

Full Name

Eukaryotic translation initiation factor 4E

Weight

25.097kDa

Superfamily

eukaryotic initiation factor 4E family

Alternative Names

CBP; eIF4E; eIF-4E; EIF4E1; EIF4EL1; EIF4EL1MGC111573; eIF-4F 25 kDa subunit; EIF4F; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein EIF4E AUTS19, CBP1, EIF4EL1, EIF4F, eIF-4E, EIF4E eukaryotic translation initiation factor 4E eukaryotic translation initiation factor 4E|eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EIF4E, check out the EIF4E Infographic

EIF4E infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EIF4E: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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2 Customer Q&As for Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody

Question

Would P00135 react with Arabidopsis samples?

Verified customer

Asked: 2019-09-14

Answer

We haven't tested the Anti-Phospho-EIF4E (S209) Rabbit Monoclonal Antibody (P00135) on Arabidopsis samples. Please run a pilot test. Immunogen sequence for this antibody is CDTATKSG-pS-TTKNRFVV.

Boster Scientific Support

Answered: 2019-09-17

Question

We are currently using anti-Phospho-eIF4E (S209) Rabbit Monoclonal antibody P00135 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

The anti-Phospho-eIF4E (S209) Rabbit Monoclonal antibody (P00135) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-15

Size

Total: $315

SKU:P00135

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P00135
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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