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Product Info Summary
| SKU: | P30930 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Yeast |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Histone H2B (T129) HIST1H2BC Rabbit Monoclonal Antibody
SKU/Catalog Number
P30930
BM4318 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H2B (T129) HIST1H2BC Rabbit Monoclonal Antibody catalog # P30930. Tested in WB application. This antibody reacts with Yeast.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H2B (T129) HIST1H2BC Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30930)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DEI-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from yeast Phospho-Histone H2B (T129)
Reactive Species
P30930 is reactive to HIST1H2BC in Yeast
Observed Molecular Weight
14 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30930 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of Histone H2B expression in Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate.
Specific Publications For Anti-Phospho-Histone H2B (T129) HIST1H2BC Rabbit Monoclonal Antibody (P30930)
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1 Customer Q&As for Anti-Phospho-Histone H2B (T129) HIST1H2BC Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-Histone H2B (T129) Rabbit Monoclonal antibody P30930 for yeast tissue, and we are content with the WB results. The species of reactivity given in the datasheet says yeast. Is it possible that the antibody can work on primate tissues as well?
A. Miller
Verified customer
Asked: 2017-06-13
Answer
The anti-Phospho-Histone H2B (T129) Rabbit Monoclonal antibody (P30930) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-13
