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Product Info Summary
| SKU: | P30442 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Phospho Histone H3.3 (Ser31) H3F3A Antibody
SKU/Catalog Number
P30442
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Histone H3.3 (Ser31) H3F3A Antibody (Catalog# P30442). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Histone H3.3 (Ser31) H3F3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30442)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Histone H3.3 around the phosphorylation site of Ser31. Immunogen sequence location: 16-65
Reactive Species
P30442 is reactive to H3F3A in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30442 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H3.3 (Phospho-Ser31) Antibody
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Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho Histone H3.3 (Ser31) H3F3A Antibody (P30442)
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Customer Q&As
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3 Customer Q&As for Anti-Phospho Histone H3.3 (Ser31) H3F3A Antibody
Question
We are currently using anti-Phospho Histone H3.3 (Ser31) antibody P30442 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
The anti-Phospho Histone H3.3 (Ser31) antibody (P30442) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-12
Question
Is this P30442 anti-Phospho Histone H3.3 (Ser31) antibody reactive to the isotypes of H3F3A?
K. Jones
Verified customer
Asked: 2017-07-19
Answer
The immunogen of P30442 anti-Phospho Histone H3.3 (Ser31) antibody is A peptide derived from human Histone H3.3 around the phosphorylation site of Ser31. Immunogen sequence location: 16-65. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-19
Question
I see that the anti-Phospho Histone H3.3 (Ser31) antibody P30442 works with IF, what is the protocol used to produce the result images on the product page?
S. Kulkarni
Verified customer
Asked: 2013-11-08
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-11-08
