Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody

Product Name

Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody

View all H3-3A Antibodies

SKU/Catalog Number

MP06819

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody catalog # MP06819. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP06819)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEO-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-Histone H3 (T3) H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

MP06819 is reactive to H3-3A in Human

Applications

MP06819 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

15.328kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of Phospho-Histone H3 (Thr3) in HeLa cell lysates treated with FBS + Calyculin A.

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Immunohistochemical analysis of paraffin-embedded human tonsil, using Phospho-Histone H3 (T3) Antibody.

Gene/Protein Information For H3-3A (Source: Uniprot.org, NCBI)

Alternative Names

Histone H3.3 H3-3A H3-3B, H3.3A, H3F3, H3F3A H3.3 histone A histone H3.3|H3 histone family member 3A|H3 histone, family 3A

For more info on H3-3A, check out the H3-3A Infographic

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In this infographic, you will see the following information for H3-3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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