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Product Info Summary
| SKU: | P05141 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho OSR1 (Thr185) Antibody
SKU/Catalog Number
P05141
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho OSR1 (Thr185) Antibody (Catalog# P05141). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho OSR1 (Thr185) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05141)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human OSR1 around the phosphorylation site of Thr185. Immunogen sequence location: 151-200
Reactive Species
P05141 is reactive to OXSR1 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
58.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P05141 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using OSR1 (Phospho-Thr185) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HepG2 cells treated with serum 20% 15', using OSR1 (Phospho-Thr185) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho OSR1 (Thr185) Antibody (P05141)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho OSR1 (Thr185) Antibody
Question
I was wanting to use your anti-Phospho OSR1 (Thr185) antibody for WB for human skeletal muscle eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skeletal muscle {eco:0000312 identification?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, P05141 anti-Phospho OSR1 (Thr185) antibody has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-30
Question
Do you have a BSA free version of anti-Phospho OSR1 (Thr185) antibody P05141 available?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho OSR1 (Thr185) antibody P05141 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-17
Question
See attached the WB image, lot number and protocol we used for skeletal muscle {eco:0000312 using anti-Phospho OSR1 (Thr185) antibody P05141. Please let me know if you require anything else.
R. Kulkarni
Verified customer
Asked: 2018-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-03
Question
We are currently using anti-Phospho OSR1 (Thr185) antibody P05141 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-27
Answer
The anti-Phospho OSR1 (Thr185) antibody (P05141) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-27
