Anti-Phospho p40 phox (Thr154) NCF4 Antibody

NCF4 antibody

Boster Bio Anti-Phospho p40 phox (Thr154) NCF4 Antibody (Catalog# P04208). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: P04208
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC-P, IF (paraffin)

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Product Name

Anti-Phospho p40 phox (Thr154) NCF4 Antibody

View all NCF4 Antibodies

SKU/Catalog Number

P04208

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho p40 phox (Thr154) NCF4 Antibody (Catalog# P04208). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho p40 phox (Thr154) NCF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04208)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

CA-20

Isotype

Rabbit IgG

Immunogen

A peptide derived from human p40 phox around the phosphorylation site of Thr154. Immunogen sequence location: 120-169

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P04208 is reactive to NCF4 in Human, Mouse

Applications

P04208 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

39.032kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500-2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For NCF4 (Source: Uniprot.org, NCBI)

Gene Name

NCF4

Full Name

Neutrophil cytosol factor 4

Weight

39.032kDa

Alternative Names

MGC3810; NCF; NCF-4; neutrophil cytosol factor 4; neutrophil cytosolic factor 4 (40kD); neutrophil cytosolic factor 4, 40kDa; Neutrophil NADPH oxidase factor 4; p40phox; p40-phox; SH3 and PX domain-containing protein 4; SH3PXD4P40PHOX NCF4 CGD3, NCF, P40PHOX, SH3PXD4 neutrophil cytosolic factor 4 neutrophil cytosol factor 4|NCF-4|SH3 and PX domain-containing protein 4|neutrophil NADPH oxidase factor 4|neutrophil cytosolic factor 4, 40kDa|p40-phox

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NCF4, check out the NCF4 Infographic

NCF4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NCF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Phospho p40 phox (Thr154) NCF4 Antibody

Question

Is a blocking peptide available for product anti-Phospho p40 phox (Thr154) antibody (P04208)?

E. Jha

Verified customer

Asked: 2018-11-30

Answer

We do provide the blocking peptide for product anti-Phospho p40 phox (Thr154) antibody (P04208). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-30

Question

We are currently using anti-Phospho p40 phox (Thr154) antibody P04208 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?

S. Roberts

Verified customer

Asked: 2018-04-04

Answer

The anti-Phospho p40 phox (Thr154) antibody (P04208) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-04

Question

My question regarding product P04208, anti-Phospho p40 phox (Thr154) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Carter

Verified customer

Asked: 2015-06-15

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04208 anti-Phospho p40 phox (Thr154) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-06-15

Order DetailsPrice
P04208

100 μL

$335

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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