Click image to see more details
Product Info Summary
| SKU: | P04208 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho p40 phox (Thr154) NCF4 Antibody
SKU/Catalog Number
P04208
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho p40 phox (Thr154) NCF4 Antibody (Catalog# P04208). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho p40 phox (Thr154) NCF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04208)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human p40 phox around the phosphorylation site of Thr154. Immunogen sequence location: 120-169
Reactive Species
P04208 is reactive to NCF4 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
39.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04208 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:5000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p40 phox (Phospho-Thr154) Antibody
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho p40 phox (Thr154) NCF4 Antibody (P04208)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho p40 phox (Thr154) NCF4 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Phospho p40 phox (Thr154) NCF4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Phospho p40 phox (Thr154) NCF4 Antibody
Question
Is a blocking peptide available for product anti-Phospho p40 phox (Thr154) antibody (P04208)?
E. Jha
Verified customer
Asked: 2018-11-30
Answer
We do provide the blocking peptide for product anti-Phospho p40 phox (Thr154) antibody (P04208). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-11-30
Question
We are currently using anti-Phospho p40 phox (Thr154) antibody P04208 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
S. Roberts
Verified customer
Asked: 2018-04-04
Answer
The anti-Phospho p40 phox (Thr154) antibody (P04208) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-04
Question
My question regarding product P04208, anti-Phospho p40 phox (Thr154) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Carter
Verified customer
Asked: 2015-06-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04208 anti-Phospho p40 phox (Thr154) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-15
