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Product Info Summary
| SKU: | P00001-4 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-p53 (S33) TP53 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00001-4
BM3987 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-p53 (S33) TP53 Rabbit Monoclonal Antibody catalog # P00001-4. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-p53 (S33) TP53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00001-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACD-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-p53 (S33)
Reactive Species
P00001-4 is reactive to TP53 in Human
Observed Molecular Weight
53 kDa
Calculated molecular weight
43.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00001-4 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: T47D cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-p53 (Ser33) in T47D cell lysates treated with etoposide.
Specific Publications For Anti-Phospho-p53 (S33) TP53 Rabbit Monoclonal Antibody (P00001-4)
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4 Customer Q&As for Anti-Phospho-p53 (S33) TP53 Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-p53 (S33) Rabbit Monoclonal antibody P00001-4 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
O. Wu
Verified customer
Asked: 2020-04-30
Answer
The anti-Phospho-p53 (S33) Rabbit Monoclonal antibody (P00001-4) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
We were satisfied with the WB result of your anti-Phospho-p53 (S33) Rabbit Monoclonal antibody. However we have observed positive staining in blood cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
According to literature, blood does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-10-08
Question
We have observed staining in human epithelium of mammary gland. Are there any suggestions? Is anti-Phospho-p53 (S33) Rabbit Monoclonal antibody supposed to stain epithelium of mammary gland positively?
M. Li
Verified customer
Asked: 2017-11-09
Answer
From what I have seen in literature epithelium of mammary gland does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2017-11-09
Question
We ordered your anti-Phospho-p53 (S33) Rabbit Monoclonal antibody for IF on ovarian adenocarcinoma in a previous experiment. I am using human, and We intend to use the antibody for WB next. you antibody examining ovarian adenocarcinoma as well as colon in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
J. Krishna
Verified customer
Asked: 2015-11-09
Answer
I viewed the website and datasheets of our anti-Phospho-p53 (S33) Rabbit Monoclonal antibody and it appears that P00001-4 has been tested on human in both IF and WB. Thus P00001-4 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-11-09
