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Product Info Summary
| SKU: | A00454T212-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-PA alpha (T212) PAK1 Antibody
SKU/Catalog Number
A00454T212-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-PA alpha (T212) PAK1 Antibody catalog # A00454T212-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PA alpha (T212) PAK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00454T212-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PAKalpha around the phosphorylation site of T212.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00454T212-1 is reactive to PAK1 in Human, Mouse, Rat
Applications
A00454T212-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
60.647kDa
Background of Pak1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-Phospho-PA alpha (T212) PAK1 Antibody A00454T212-1
Western Blot (WB) analysis of 293 cells using Phospho-PAKalpha (T212) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PAK1 (Source: Uniprot.org, NCBI)
Gene Name
PAK1
Full Name
Serine/threonine-protein kinase PAK 1
Weight
60.647kDa
Superfamily
protein kinase superfamily
Alternative Names
alpha-PAK; EC 2.7.11; EC 2.7.11.1; MGC130000; MGC130001; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21-activated kinase 1; p65-PAK; PAK1; PAK-1; PAKalpha; serine/threonine-protein kinase PAK 1; STE20 homolog, yeast PAK1 IDDMSSD, PAKalpha, alpha-PAK, p65-PAK p21 (RAC1) activated kinase 1 serine/threonine-protein kinase PAK 1|STE20 homolog, yeast|p21 protein (Cdc42/Rac)-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAK1, check out the PAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PA alpha (T212) PAK1 Antibody (A00454T212-1)
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No publications found for A00454T212-1
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