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Product Info Summary
| SKU: | MP01723 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody
SKU/Catalog Number
MP01723
BM5435 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody catalog # MP01723. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP01723)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADFF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival.
Reactive Species
MP01723 is reactive to PAK4 in Human, Mouse, Rat
Observed Molecular Weight
64, 80, 75 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP01723 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:200
Positive Control
WB: U87-MG cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) expression in U87-MG cell lysate.
Specific Publications For Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody (MP01723)
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7 Customer Q&As for Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody
Question
Do you have a BSA free version of anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
S. Jones
Verified customer
Asked: 2019-01-31
Answer
The anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody (MP01723) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-31
Question
I was wanting to use your anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody for WB for mouse colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon carcinoma identification?
J. Jones
Verified customer
Asked: 2018-10-18
Answer
It shows on the product datasheet, MP01723 anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-18
Question
Is this MP01723 anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody reactive to the isotypes of PAK4?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
The immunogen of MP01723 anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody is A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-22
Question
Does anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 work for WB with colon carcinoma?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
According to the expression profile of colon carcinoma, PAK4 is highly expressed in colon carcinoma. So, it is likely that anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 will work for WB with colon carcinoma.
Boster Scientific Support
Answered: 2018-01-03
Question
Could you ask Abways if they could provide additional western blot data comparing lysate from treated vs untreated cells? For the following antibodies: MP01723 | CY7154 P03124 | CY5349 MP04922 | CY5663 P00732 | CY5256 MP00027 | CY6570 The customer said "It would be great if there was data showing weak or no band in untreated and stronger band in treated. This would demonstrate that the treatment was the cause of the band intensity difference."
Verified Customer
Verified customer
Asked: 2016-09-15
Answer
Abways doesn't have the additional western blot data comparing lysate from treated vs untreated cells.
Boster Scientific Support
Answered: 2016-09-15
Question
I see that the anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 works with WB, what is the protocol used to produce the result images on the product page?
K. Krishna
Verified customer
Asked: 2015-05-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-05-06
