Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody

Boster Bio Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody catalog # MP01723. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: MP01723
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody

See all PAK4 products

SKU/Catalog Number

MP01723

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody catalog # MP01723. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP01723)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADFF-16

Immunogen

A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

MP01723 is reactive to PAK4 in Human, Mouse, Rat

Applications

MP01723 is guaranteed for Flow Cytometry, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:2000 FC 1:200

Validation Images & Assay Conditions

Gene/Protein Information For PAK4 (Source: Uniprot.Org, NCBI)

Uniprot ID

O96013

Gene ID

10298

Gene Name

PAK4

Full Name

Serine/threonine-protein kinase PAK 4

Weight

64.072kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.1; KIAA1142; p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; PAK4; PAK-4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; serine/threonine-protein kinase PAK 4 PAK4 p21 (RAC1) activated kinase 4 serine/threonine-protein kinase PAK 4|p21 protein (Cdc42/Rac)-activated kinase 4|p21(CDKN1A)-activated kinase 4|protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PAK4, check out the PAK4 Infographic

PAK4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PAK4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody

Question

Do you have a BSA free version of anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 available?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-29

Question

We are currently using anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

S. Jones

Verified customer

Asked: 2019-01-31

Answer

The anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody (MP01723) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-31

Question

I was wanting to use your anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody for WB for mouse colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon carcinoma identification?

J. Jones

Verified customer

Asked: 2018-10-18

Answer

It shows on the product datasheet, MP01723 anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-18

Question

Is this MP01723 anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody reactive to the isotypes of PAK4?

Verified Customer

Verified customer

Asked: 2018-08-22

Answer

The immunogen of MP01723 anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody is A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-22

Question

Does anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 work for WB with colon carcinoma?

Verified Customer

Verified customer

Asked: 2018-01-03

Answer

According to the expression profile of colon carcinoma, PAK4 is highly expressed in colon carcinoma. So, it is likely that anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 will work for WB with colon carcinoma.

Boster Scientific Support

Answered: 2018-01-03

Question

Could you ask Abways if they could provide additional western blot data comparing lysate from treated vs untreated cells? For the following antibodies: MP01723 | CY7154 P03124 | CY5349 MP04922 | CY5663 P00732 | CY5256 MP00027 | CY6570 The customer said "It would be great if there was data showing weak or no band in untreated and stronger band in treated. This would demonstrate that the treatment was the cause of the band intensity difference."

Verified Customer

Verified customer

Asked: 2016-09-15

Answer

Abways doesn't have the additional western blot data comparing lysate from treated vs untreated cells.

Boster Scientific Support

Answered: 2016-09-15

Question

I see that the anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal antibody MP01723 works with WB, what is the protocol used to produce the result images on the product page?

K. Krishna

Verified customer

Asked: 2015-05-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-05-06

Size

Total: $315

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
Order Product
MP01723
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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