Product Info Summary
SKU: | A02623T9 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-PBK (T9) Antibody
SKU/Catalog Number
A02623T9
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-PBK (T9) Antibody catalog # A02623T9. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PBK (T9) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02623T9)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PBK around the phosphorylation site of T9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02623T9 is reactive to Pbk in Human
Applications
A02623T9 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.745kDa
Background of Pbk
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.
Tsai L.-H., Nature 371:419-423(1994).
Patrick G.N., Nature 402:615-622(1999).
Kesavapany S., J. Neurosci. 24:4421-4431(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-PBK (T9) Antibody A02623T9
Western Blot (WB) analysis of 293T cells using Phospho-PBK (T9) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Pbk (Source: Uniprot.org, NCBI)
Gene Name
Pbk
Full Name
Lymphokine-activated killer T-cell-originated protein kinase
Weight
36.745kDa
Superfamily
protein kinase superfamily
Alternative Names
Cancer/testis antigen 84; CT84MAPKK-like protein kinase; EC 2.7.12.2; FLJ14385; Nori-3PDZ-binding kinase; PDZ binding kinase; serine/threonine protein kinase; Spermatogenesis-related protein kinase; SPKT-LAK cell-originated protein kinase; TOPKlymphokine-activated killer T-cell-originated protein kinase Pbk|2810434B10Rik, AW538537, D14Ertd732, D14Ertd732e, TO, TOPK|PDZ binding kinase|lymphokine-activated killer T-cell-originated protein kinase|MAPKK-like protein kinase|T-LAK cell-originated protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pbk, check out the Pbk Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pbk: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PBK (T9) Antibody (A02623T9)
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No publications found for A02623T9
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