Product Info Summary
SKU: | P00096 |
---|---|
Size: | 100 μl |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody
View all PDGF R beta Antibodies
SKU/Catalog Number
P00096
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody catalog # P00096. Tested in WB, ICC/IF applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00096)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FDF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-PDGF Receptor beta (Y740)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00096 is reactive to PDGFRB in Mouse, Rat
Applications
P00096 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
100 kDa
Calculated molecular weight
123.968kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phospho-PDGF Receptor beta (Y740) expression in NIH 3T3 treated with PDGF.
Protein Target Info & Infographic
Gene/Protein Information For PDGFRB (Source: Uniprot.org, NCBI)
Gene Name
PDGFRB
Full Name
Platelet-derived growth factor receptor beta
Weight
123.968kDa
Superfamily
protein kinase superfamily
Alternative Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide PDGFRB CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT platelet derived growth factor receptor beta platelet-derived growth factor receptor beta|Activated tyrosine kinase PDGFRB|CD140 antigen-like family member B|NDEL1-PDGFRB|PDGF-R-beta|PDGFR-beta|beta-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 1|platelet-derived growth factor receptor, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDGFRB, check out the PDGFRB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDGFRB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody (P00096)
Hello CJ!
P00096 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
RNA interference targeting the platelet-derived growth factor receptor ? subunit ameliorates experimental hepatic fibrosis in rats
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody
Question
We have seen staining in mouse brain. Any tips? Is anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
According to literature brain does express PDGFRB. According to Uniprot.org, PDGFRB is expressed in ectocervix, brain, among other tissues. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-08
Question
We are currently using anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody P00096 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody (P00096) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
My colleagues were satisfied with the WB result of your anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody. However we have seen positive staining in ectocervix cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Based on literature, ectocervix does express PDGFRB. Generally PDGFRB expresses in cell membrane. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-29
Question
Our lab used your anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody for IF on ectocervix in a previous project. I am using rat, and I plan to use the antibody for WB next. We are interested in examining ectocervix as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for WB?
C. Jha
Verified customer
Asked: 2013-08-23
Answer
I viewed the website and datasheets of our anti-Phospho-PDGF Receptor beta (Y740) Rabbit Monoclonal antibody and it appears that P00096 has been validated on rat in both IF and WB. Thus P00096 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-08-23