Anti-Phospho-PLK1 (T210) Rabbit Monoclonal Antibody

Plk1 antibody

Boster Bio Anti-Phospho-PLK1 (T210) Rabbit Monoclonal Antibody catalog # P00182-2. Tested in WB application. This antibody reacts with Human.

Product Info Summary

SKU: P00182-2
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-PLK1 (T210) Rabbit Monoclonal Antibody

View all Plk1 Antibodies

SKU/Catalog Number

P00182-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-PLK1 (T210) Rabbit Monoclonal Antibody catalog # P00182-2. Tested in WB application. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-PLK1 (T210) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00182-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HAO-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PLK1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00182-2 is reactive to PLK1 in Human

Applications

P00182-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

68.255kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PLK1 (Source: Uniprot.org, NCBI)

Gene Name

PLK1

Full Name

Serine/threonine-protein kinase PLK1

Weight

68.255kDa

Superfamily

protein kinase superfamily

Alternative Names

cell cycle regulated protein kinase; EC 2.7.11; EC 2.7.11.21; PLK; PLK1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase 1polo-like kinase (Drosophila); Serine/threonine-protein kinase 13; serine/threonine-protein kinase PLK1; STPK13 PLK1 PLK, STPK13 polo like kinase 1 serine/threonine-protein kinase PLK1|PLK-1|cell cycle regulated protein kinase|polo (Drosophia)-like kinase|serine/threonine-protein kinase 13

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PLK1, check out the PLK1 Infographic

PLK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PLK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-Phospho-PLK1 (T210) Rabbit Monoclonal Antibody

Question

Is this P00182-2 anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody reactive to the isotypes of PLK1?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

The immunogen of P00182-2 anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody is A synthesized peptide derived from human PLK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-05-08

Question

I see that the anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-31

Question

I was wanting to use your anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody for WB for human colon lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon lung identification?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

As indicated on the product datasheet, P00182-2 anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-21

Question

I am interested in to test anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 on human colon lung for research purposes, then I may be interested in using anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

The products we sell, including anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-25

Question

We are currently using anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody (P00182-2) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-19

Question

Would anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 work for WB with colon lung?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

According to the expression profile of colon lung, PLK1 is highly expressed in colon lung. So, it is likely that anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 will work for WB with colon lung.

Boster Scientific Support

Answered: 2019-07-26

Question

I am looking for using your anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody for synaptonemal complex disassembly studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We appreciate your inquiry. This P00182-2 anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-07

Question

We have seen staining in human colon lung. Are there any suggestions? Is anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody supposed to stain colon lung positively?

G. Edwards

Verified customer

Asked: 2018-10-15

Answer

Based on literature colon lung does express PLK1. Based on Uniprot.org, PLK1 is expressed in testis, placenta, lung, colon lung, lymphoblast, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PLK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, and Lung, Pubmed ID: 15489334
Lung, Pubmed ID: 8127874
Lymphoblast, Pubmed ID: 14654843
Placenta, Pubmed ID: 8018557, 7902533

Boster Scientific Support

Answered: 2018-10-15

Question

Do you have a BSA free version of anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 available?

G. Carter

Verified customer

Asked: 2018-04-10

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-04-10

Question

Would P00182-2 anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Walker

Verified customer

Asked: 2018-02-19

Answer

As indicated on the product datasheet, P00182-2 anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-19

Question

Is a blocking peptide available for product anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody (P00182-2)?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

We do provide the blocking peptide for product anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody (P00182-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-04

Question

My team were well pleased with the WB result of your anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody. However we have been able to see positive staining in lymphoblast centromere, using this antibody. Is that expected? Could you tell me where is PLK1 supposed to be expressed?

J. Singh

Verified customer

Asked: 2016-05-10

Answer

From what I have seen in literature, lymphoblast does express PLK1. Generally PLK1 expresses in nucleus. chromosome, centromere,. Regarding which tissues have PLK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, and Lung, Pubmed ID: 15489334
Lung, Pubmed ID: 8127874
Lymphoblast, Pubmed ID: 14654843
Placenta, Pubmed ID: 8018557, 7902533

Boster Scientific Support

Answered: 2016-05-10

Question

See below the WB image, lot number and protocol we used for colon lung using anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2. Please let me know if you require anything else.

J. Li

Verified customer

Asked: 2015-05-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-05-13

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon lung using anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody P00182-2. Let me know if you need anything else.

D. Carter

Verified customer

Asked: 2014-07-28

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-07-28

Question

My question regarding product P00182-2, anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Li

Verified customer

Asked: 2013-08-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00182-2 anti-Phospho-PLK1 (T210) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-08-07

Order DetailsPrice
P00182-2

100μL

$370
P00182-2-30ul

30μL sample

$99
P00182-2-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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