Anti-Phospho-Rad17 (Ser646) Polyclonal Antibody

Product Name

Anti-Phospho-Rad17 (Ser646) Polyclonal Antibody

View all RAD17 Antibodies

SKU/Catalog Number

P02159

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Rad17 (Ser646) Polyclonal Antibody (Catalog # P02159). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Rad17 (Ser646) Polyclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02159)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from human Rad17 around the phosphorylation site of S646.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P02159 is reactive to RAD17 in Human, Mouse, Rat

Applications

P02159 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

77.055kDa

Background of RAD17

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Essers MA, et al. EMBO J 2004 Nov. 11.
Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.
Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1/500 - 1/2000
ELISA: 1/10000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Western Blot analysis of K562, cells using Phospho-Rad17 (S646) Polyclonal antibody

Gene/Protein Information For RAD17 (Source: Uniprot.org, NCBI)

Alternative Names

CCYC; cell cycle checkpoint protein (RAD17); cell cycle checkpoint protein RAD17; FLJ41520; HRAD17; R24L; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17 homolog (S. pombe); Rad17; Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog RAD17 CCYC, HRAD17, R24LSP, RAD24, RAD17 RAD17 checkpoint clamp loader component cell cycle checkpoint protein RAD17|RAD1 homolog|RAD17 homolog|RF-C activator 1 homolog|Rad17-like protein|cell cycle checkpoint protein (RAD17)

For more info on RAD17, check out the RAD17 Infographic

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In this infographic, you will see the following information for RAD17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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