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Product Info Summary
| SKU: | A00446S621-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Raf-1 (S621) Antibody
SKU/Catalog Number
A00446S621-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Raf-1 (S621) Antibody catalog # A00446S621-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Raf-1 (S621) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00446S621-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Raf-1 around the phosphorylation site of S621.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00446S621-2 is reactive to RAF1 in Human, Monkey, Mouse, Rat
Applications
A00446S621-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
73.052kDa
Background of Raf-1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For RAF1 (Source: Uniprot.org, NCBI)
Gene Name
RAF1
Full Name
RAF proto-oncogene serine/threonine-protein kinase
Weight
73.052kDa
Superfamily
protein kinase superfamily
Alternative Names
cRaf; c-Raf; c-Raf-1; CRAFraf proto-oncogene serine/threonine protein kinase; EC 2.7.11; EC 2.7.11.1; NS5; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; RAF; Raf1; Raf-1; Raf-1Oncogene RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; v-Raf-1 RAF1 CMD1NN, CRAF, NS5, Raf-1, c-Raf Raf-1 proto-oncogene, serine/threonine kinase RAF proto-oncogene serine/threonine-protein kinase|C-Raf proto-oncogene, serine/threonine kinase|Oncogene RAF1|proto-oncogene c-RAF|raf proto-oncogene serine/threonine protein kinase|v-raf-1 murine leukemia viral oncogene homolog 1|v-raf-1 murine leukemia viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAF1, check out the RAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Raf-1 (S621) Antibody (A00446S621-2)
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No publications found for A00446S621-2
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