Click image to see more details
Product Info Summary
| SKU: | P03338 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho Ras-GRF1 (Ser916) Antibody
SKU/Catalog Number
P03338
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Ras-GRF1 (Ser916) Antibody (Catalog# P03338). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Ras-GRF1 (Ser916) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03338)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from mouse Ras-GRF1 around the phosphorylation site of Ser916. Immunogen sequence location: 882-931
Reactive Species
P03338 is reactive to Rasgrf1 in Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
144.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03338 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ras-GRF1 (Phospho-Ser916) Antibody
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using Ras-GRF1 (Phospho-Ser916) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho Ras-GRF1 (Ser916) Antibody (P03338)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho Ras-GRF1 (Ser916) Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Phospho Ras-GRF1 (Ser916) Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Phospho Ras-GRF1 (Ser916) Antibody
Question
I was wanting to use your anti-Phospho Ras-GRF1 (Ser916) antibody for IHC for mouse c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse c1 segment of cervical spinal cord identification?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
You can see on the product datasheet, P03338 anti-Phospho Ras-GRF1 (Ser916) antibody has been tested for IHC on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-12
Question
We are currently using anti-Phospho Ras-GRF1 (Ser916) antibody P03338 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-Phospho Ras-GRF1 (Ser916) antibody (P03338) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
Would anti-Phospho Ras-GRF1 (Ser916) antibody P03338 work on horse IHC with brain?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Our lab technicians have not tested anti-Phospho Ras-GRF1 (Ser916) antibody P03338 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Phospho Ras-GRF1 (Ser916) antibody P03338 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-08
Question
Is this P03338 anti-Phospho Ras-GRF1 (Ser916) antibody reactive to the isotypes of RASGRF1?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The immunogen of P03338 anti-Phospho Ras-GRF1 (Ser916) antibody is A peptide derived from mouse Ras-GRF1 around the phosphorylation site of Ser916. Immunogen sequence location: 882-931. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-Phospho Ras-GRF1 (Ser916) antibody P03338. Let me know if you need anything else.
P. Johnson
Verified customer
Asked: 2017-09-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-20
Question
Do you have a BSA free version of anti-Phospho Ras-GRF1 (Ser916) antibody P03338 available?
J. Baker
Verified customer
Asked: 2014-06-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho Ras-GRF1 (Ser916) antibody P03338 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-06-19
Question
Does P03338 anti-Phospho Ras-GRF1 (Ser916) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Moore
Verified customer
Asked: 2013-08-28
Answer
It shows on the product datasheet, P03338 anti-Phospho Ras-GRF1 (Ser916) antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-28
