Anti-Phospho Ras-GRF1 (Ser916) Antibody

Boster Bio Anti-Phospho Ras-GRF1 (Ser916) Antibody catalog # P03338. Tested in IHC applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: P03338
Size: 100ul
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC

Product Name

Anti-Phospho Ras-GRF1 (Ser916) Antibody

See all Rasgrf1 products

SKU/Catalog Number

P03338

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho Ras-GRF1 (Ser916) Antibody catalog # P03338. Tested in IHC applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho Ras-GRF1 (Ser916) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03338)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

A peptide derived from mouse Ras-GRF1 around the phosphorylation site of Ser916. Immunogen sequence location: 882-931

Reactive Species

P03338 is reactive to Rasgrf1 in Mouse, Rat

Applications

P03338 is guaranteed for IHC Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For Rasgrf1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P27671

Gene ID

19417

Gene Name

Rasgrf1

Full Name

Ras-specific guanine nucleotide-releasing factor 1

Weight

144.102kDa

Alternative Names

CDC25guanine nucleotide exchange factor; CDC25L; GNRPguanine nucleotide-releasing factor 1; GRF1; GRF55; guanine nucleotide-releasing factor, 55 kD; Guanine nucleotide-releasing protein; H-GRF55; PP13187; Ras protein-specific guanine nucleotide-releasing factor 1; ras-GRF1; ras-specific guanine nucleotide-releasing factor 1; Ras-specific guanine nucleotide-releasing factor, CDC25 homolog; Ras-specific nucleotide exchange factor CDC25 Rasgrf1|AI844718, CDC25, CDC25Mm, Gnrp, Grf, Grf1, Grfbe, Grfbeta, P190-A, Ras-GRF1, p190, p190RhoGEF|RAS protein-specific guanine nucleotide-releasing factor 1|ras-specific guanine nucleotide-releasing factor 1|Ras guanine release factor beta|ras-specific nucleotide exchange factor CDC25

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Rasgrf1, check out the Rasgrf1 Infographic

Rasgrf1 infographic

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In this infographic you will see the following information for Rasgrf1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-Phospho Ras-GRF1 (Ser916) Antibody

Question

I was wanting to use your anti-Phospho Ras-GRF1 (Ser916) antibody for IHC for mouse c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse c1 segment of cervical spinal cord identification?

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

You can see on the product datasheet, P03338 anti-Phospho Ras-GRF1 (Ser916) antibody has been tested for IHC on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-12

Question

We are currently using anti-Phospho Ras-GRF1 (Ser916) antibody P03338 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

The anti-Phospho Ras-GRF1 (Ser916) antibody (P03338) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-13

Question

Would anti-Phospho Ras-GRF1 (Ser916) antibody P03338 work on horse IHC with brain?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

Our lab technicians have not tested anti-Phospho Ras-GRF1 (Ser916) antibody P03338 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Phospho Ras-GRF1 (Ser916) antibody P03338 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-08

Question

Is this P03338 anti-Phospho Ras-GRF1 (Ser916) antibody reactive to the isotypes of RASGRF1?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

The immunogen of P03338 anti-Phospho Ras-GRF1 (Ser916) antibody is A peptide derived from mouse Ras-GRF1 around the phosphorylation site of Ser916. Immunogen sequence location: 882-931. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-05

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-Phospho Ras-GRF1 (Ser916) antibody P03338. Let me know if you need anything else.

P. Johnson

Verified customer

Asked: 2017-09-20

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-20

Question

Do you have a BSA free version of anti-Phospho Ras-GRF1 (Ser916) antibody P03338 available?

J. Baker

Verified customer

Asked: 2014-06-19

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho Ras-GRF1 (Ser916) antibody P03338 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-06-19

Question

Does P03338 anti-Phospho Ras-GRF1 (Ser916) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Moore

Verified customer

Asked: 2013-08-28

Answer

It shows on the product datasheet, P03338 anti-Phospho Ras-GRF1 (Ser916) antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-08-28

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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P03338
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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