Click image to see more details
Product Info Summary
| SKU: | P30435 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Phospho Rhodopsin (Ser334) Antibody
SKU/Catalog Number
P30435
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Rhodopsin (Ser334) Antibody (Catalog# P30435). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Rhodopsin (Ser334) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30435)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Rhodopsin around the phosphorylation site of Ser334. Immunogen sequence location: 299-348
Reactive Species
P30435 is reactive to RHO in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
38.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30435 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rhodopsin (Phospho-Ser334) Antibody
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using Rhodopsin (Phospho-Ser334) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho Rhodopsin (Ser334) Antibody (P30435)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho Rhodopsin (Ser334) Antibody
Question
Would anti-Phospho Rhodopsin (Ser334) antibody P30435 work on pig IHC with retina?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Our lab technicians have not tested anti-Phospho Rhodopsin (Ser334) antibody P30435 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Phospho Rhodopsin (Ser334) antibody P30435 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig retina in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-23
Question
I see that the anti-Phospho Rhodopsin (Ser334) antibody P30435 works with IHC, what is the protocol used to produce the result images on the product page?
M. Zhang
Verified customer
Asked: 2019-07-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-10
Question
I am interested in to test anti-Phospho Rhodopsin (Ser334) antibody P30435 on human retina for research purposes, then I may be interested in using anti-Phospho Rhodopsin (Ser334) antibody P30435 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The products we sell, including anti-Phospho Rhodopsin (Ser334) antibody P30435, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-09
Question
We are currently using anti-Phospho Rhodopsin (Ser334) antibody P30435 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
The anti-Phospho Rhodopsin (Ser334) antibody (P30435) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-17
Question
Would anti-Phospho Rhodopsin (Ser334) antibody P30435 work for IHC with retina?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
According to the expression profile of retina, RHO is highly expressed in retina. So, it is likely that anti-Phospho Rhodopsin (Ser334) antibody P30435 will work for IHC with retina.
Boster Scientific Support
Answered: 2018-12-26
