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Product Info Summary
| SKU: | A04364S801 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Separase (S801) ESPL1 Antibody
SKU/Catalog Number
A04364S801
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Separase (S801) ESPL1 Antibody catalog # A04364S801. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Separase (S801) ESPL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04364S801)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801. AA range:767-816
Reactive Species
A04364S801 is reactive to ESPL1 in Human, Mouse
Calculated molecular weight
233.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04364S801 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Separase (S801) Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SEPARASE (Phospho-Ser801) Antibody
Specific Publications For Anti-Phospho-Separase (S801) ESPL1 Antibody (A04364S801)
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