Anti-Phospho-Sgo1 (S14) Antibody

Product Name

Anti-Phospho-Sgo1 (S14) Antibody

View all Shugoshin Antibodies

SKU/Catalog Number

A03083S14

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-Sgo1 (S14) Antibody catalog # A03083S14. Tested in ELISA, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Sgo1 (S14) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03083S14)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Sgo1 around the phosphorylation site of S14.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03083S14 is reactive to Sgo1 in Human

Applications

A03083S14 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

58.966kDa

Background of Shugoshin

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.

McGuinness B.E., PLoS Biol. 3:433-449(2005).
Kitajima T.S., Curr. Biol. 15:353-359(2005).
Tang Z., Proc. Natl. Acad. Sci. U.S.A. 101:18012-18017(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:100-1:300
ELISA 1:5000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For Sgo1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ14230; hSgo1; NY-BR-85; Serologically defined breast cancer antigen NY-BR-85; SGO; Sgo1; shugoshin 1AB protein; shugoshin 1CD protein; shugoshin 1EF protein; shugoshin 1GH protein; shugoshin 1KL protein; shugoshin-like 1 (S. pombe); shugoshin-like 1 Sgo1|3300001M08Rik, C81037, Sg, Sgol1|shugoshin 1|shugoshin 1|shugoshin 1A protein|shugoshin-like 1

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In this infographic, you will see the following information for Sgo1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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