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Product Info Summary
| SKU: | P05439 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho TIF-IA (Ser649) Antibody
SKU/Catalog Number
P05439
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho TIF-IA (Ser649) Antibody (Catalog# P05439). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho TIF-IA (Ser649) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05439)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TIF-IA around the phosphorylation site of Ser649. Immunogen sequence location: 602-651
Reactive Species
P05439 is reactive to RRN3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
74.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P05439 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIF-IA (Phospho-Ser649) Antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from Jurkat cells treated with starved 24h, using TIF-IA (Phospho-Ser649) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho TIF-IA (Ser649) Antibody (P05439)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Phospho TIF-IA (Ser649) Antibody
Question
Does P05439 anti-Phospho TIF-IA (Ser649) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
You can see on the product datasheet, P05439 anti-Phospho TIF-IA (Ser649) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-27
Question
We are currently using anti-Phospho TIF-IA (Ser649) antibody P05439 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
The anti-Phospho TIF-IA (Ser649) antibody (P05439) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-28
Question
Is this P05439 anti-Phospho TIF-IA (Ser649) antibody reactive to the isotypes of RRN3?
L. Krishna
Verified customer
Asked: 2016-10-12
Answer
The immunogen of P05439 anti-Phospho TIF-IA (Ser649) antibody is A peptide derived from human TIF-IA around the phosphorylation site of Ser649. Immunogen sequence location: 602-651. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-10-12
