Anti-Phospho TIF-IA (Ser649) Antibody

Boster Bio Anti-Phospho TIF-IA (Ser649) Antibody catalog # P05439. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: P05439
Size: 100ul
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Phospho TIF-IA (Ser649) Antibody

See all RRN3 products

SKU/Catalog Number

P05439

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho TIF-IA (Ser649) Antibody catalog # P05439. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho TIF-IA (Ser649) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05439)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

A peptide derived from human TIF-IA around the phosphorylation site of Ser649. Immunogen sequence location: 602-651

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P05439 is reactive to RRN3 in Human

Applications

P05439 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For RRN3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NYV6

Gene ID

54700

Gene Name

RRN3

Full Name

RNA polymerase I-specific transcription initiation factor RRN3

Weight

74.107kDa

Superfamily

RRN3 family

Alternative Names

DKFZp566E104; RNA polymerase I-specific transcription initiation factor RRN3; RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae); RRN3 RNA polymerase I transcription factor homolog (yeast); TIF-IA; TIFIAMGC104238; Transcription initiation factor IA; transcription initiation factor TIF-IA RRN3 A-270G1.2, TIFIA RRN3 homolog, RNA polymerase I transcription factor RNA polymerase I-specific transcription initiation factor RRN3|RRN3 RNA polymerase I transcription factor homolog|TIF-IA|transcription initiation factor IA|transcription initiation factor TIF-IA

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RRN3, check out the RRN3 Infographic

RRN3 infographic

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In this infographic you will see the following information for RRN3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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3 Customer Q&As for Anti-Phospho TIF-IA (Ser649) Antibody

Question

Does P05439 anti-Phospho TIF-IA (Ser649) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

You can see on the product datasheet, P05439 anti-Phospho TIF-IA (Ser649) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-27

Question

We are currently using anti-Phospho TIF-IA (Ser649) antibody P05439 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-28

Answer

The anti-Phospho TIF-IA (Ser649) antibody (P05439) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-28

Question

Is this P05439 anti-Phospho TIF-IA (Ser649) antibody reactive to the isotypes of RRN3?

L. Krishna

Verified customer

Asked: 2016-10-12

Answer

The immunogen of P05439 anti-Phospho TIF-IA (Ser649) antibody is A peptide derived from human TIF-IA around the phosphorylation site of Ser649. Immunogen sequence location: 602-651. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-10-12

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
P05439
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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