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- Table of Contents
YAP1 antibody (P00116) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Immunohistochemical analysis of paraffin-embedded human stomach, using Phospho-YAP1 (S127) Antibody.
Designing a Western blot for YAP1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the YAP1 Western Blot GuideBundle Save
Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody
YAP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
YAP1 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, rat RH35 whole cell, mouse HEPA1-6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
YAP1 antibody was positively detected by IHC in:
human stomach tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody
View all YAP1 Antibodies
View all YAP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GAB-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human YAP1
Observed Molecular Weight
70, 75 kDa
Calculated Molecular Weight
54.5 kDa
Cite This Product
Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00116)
Protocols
Western Blot protocol for YAP1 antibody
Immunohistochemistry (IHC) protocol for YAP1 antibody
Publications
Application:
Species:
KCNJ15 inhibits chemical-induced lung carcinogenesis and progression through GNB1 mediated Hippo pathway
Published: 2024-12-25
Journal: TOXICOLOGY
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 39725264
Dapagliflozin delays renal fibrosis in diabetic kidney disease by inhibiting YAP/TAZ activation
Published: 2023-04-05
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 6.10
Species: Human,Mouse
PMID: 37023953
Shear stress inhibits cardiac microvascular endothelial cells apoptosis to protect against myocardial ischemia reperfusion injury via YAP/miR-206/PDCD4 signaling pathway
Published: 2021-02-18
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.86
Species: Rat
PMID: 33610591
YAP accelerates vascular senescence via blocking autophagic flux and activating mTOR
Published: 2020-12-12
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.49
Species: Rat,Human
PMID: 33314583
PMMA Bone Cement Acts on the Hippo/YAP Pathway To Regulate CTGF and Induce Intervertebral Disc Degeneration
Published: 2019-05-23
Journal: ACS Biomaterials Science & Engineering
Application: WB
Impact Factor: 4.51
Species: Human
PMID: 33405572
Review
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Product Promise
We promise Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00116
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Nov 1, 2026