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Product Info Summary
| SKU: | A10773 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PI 3-Kinase C2 gamma PIK3C2G Antibody
SKU/Catalog Number
A10773
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PI 3-Kinase C2 gamma PIK3C2G Antibody catalog # A10773. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3-Kinase C2 gamma PIK3C2G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10773)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the N-terminal region of human PI 3-Kinase C2γ.
Reactive Species
A10773 is reactive to PIK3C2G in Human, Mouse, Rat
Calculated molecular weight
165.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10773 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western blot analysis of A549 using PI 3-Kinase C2γ antibody. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-PI 3-Kinase C2 gamma PIK3C2G Antibody (A10773)
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Customer Q&As
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6 Customer Q&As for Anti-PI 3-Kinase C2 gamma PIK3C2G Antibody
Question
Will anti-PI 3-Kinase C2 gamma antibody A10773 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
According to the expression profile of liver, PIK3C2G is highly expressed in liver. So, it is likely that anti-PI 3-Kinase C2 gamma antibody A10773 will work for WB with liver.
Boster Scientific Support
Answered: 2020-05-05
Question
Would A10773 anti-PI 3-Kinase C2 gamma antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can see on the product datasheet, A10773 anti-PI 3-Kinase C2 gamma antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-24
Question
We are currently using anti-PI 3-Kinase C2 gamma antibody A10773 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
The anti-PI 3-Kinase C2 gamma antibody (A10773) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PI 3-Kinase C2 gamma antibody A10773. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-21
Question
Will anti-PI 3-Kinase C2 gamma antibody A10773 work on primate WB with liver?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
Our lab technicians have not validated anti-PI 3-Kinase C2 gamma antibody A10773 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PI 3-Kinase C2 gamma antibody A10773 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-24
Question
We are interested in to test anti-PI 3-Kinase C2 gamma antibody A10773 on human liver for research purposes, then I may be interested in using anti-PI 3-Kinase C2 gamma antibody A10773 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Yang
Verified customer
Asked: 2016-08-09
Answer
The products we sell, including anti-PI 3-Kinase C2 gamma antibody A10773, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-08-09
