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Product Info Summary
| SKU: | M06707 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody
View all PI 3-Kinase p55 gamma Antibodies
SKU/Catalog Number
M06707
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody catalog # M06707. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06707)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DHC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PI 3 Kinase p55 gamma
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06707 is reactive to PIK3R3 in Human, Mouse, Rat
Applications
M06707 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
130 kDa, 270 kDa
Calculated molecular weight
54.448kDa
Background of PI 3-Kinase p55 gamma
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of PI 3 Kinase p55 gamma expression in SH-SY5Y cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:800 dilution.
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Immunohistochemical analysis of paraffin-embedded human astrocytoma, using PI 3 Kinase p55 gamma Antibody.
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Immunofluorescent analysis of C6 cells, using PI 3 Kinase p55 gamma Antibody .
Protein Target Info & Infographic
Gene/Protein Information For PIK3R3 (Source: Uniprot.org, NCBI)
Gene Name
PIK3R3
Full Name
Phosphatidylinositol 3-kinase regulatory subunit gamma
Weight
54.448kDa
Superfamily
PI3K p85 subunit family
Alternative Names
100% homology to SWISS-PROT Q92569; DKFZp686P05226; FLJ41892; p55; p55PIK; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphoinositide-3-kinase, regulatory subunit 3 (gamma); phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); PI 3Kinase p55 gamma; PI 3-Kinase p55 gamma; PI3K regulatory subunit gamma; PI3-kinase subunit p55-gamma; PIK3R3; regulatory subunit, polypeptide 3 (p55, gamma) PIK3R3 p55, p55-GAMMA, p55PIK phosphoinositide-3-kinase regulatory subunit 3 phosphatidylinositol 3-kinase regulatory subunit gamma|PI3-kinase regulatory subunit gamma|PI3-kinase subunit p55-gamma|PI3K regulatory subunit gamma|phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma)|phosphoinositide-3-kinase, regulatory subunit 3 (gamma)|ptdIns-3-kinase regulatory subunit p55-gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIK3R3, check out the PIK3R3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIK3R3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody (M06707)
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No publications found for M06707
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody (M06707)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We do provide the blocking peptide for product anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody (M06707). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-18
Question
Would anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 work on bovine WB with nasal cavity epithelium?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Our lab technicians have not tested anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine nasal cavity epithelium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
We are currently using anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
T. Banerjee
Verified customer
Asked: 2015-04-16
Answer
The anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody (M06707) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-16
