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Product Info Summary
| SKU: | M06618-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PI 3 Kinase R4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06618-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-PI 3 Kinase R4 Rabbit Monoclonal Antibody catalog # M06618-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 Kinase R4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06618-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
25P13
Isotype
IgG
Immunogen
A synthesized peptide derived from human PI 3 Kinase R4
Reactive Species
M06618-1 is reactive to PIK3R4 in Human, Mouse, Rat
Observed Molecular Weight
150 kDa
Calculated molecular weight
153.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06618-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: Jurkat cell, Molt-4 cell
IHC: human liver tissue, human liver cancer tissue, rat kidney tissue
IHC: human liver tissue, human liver cancer tissue, rat kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of PI 3 Kinase R4 expression in (1) Jurkat cell lysate; (2) Molt-4 cell lysate.
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IHC analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (M06618-1).
VPS15/PIK3R4 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-VPS15/PIK3R4 Antibody (M06618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (M06618-1).
VPS15/PIK3R4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-VPS15/PIK3R4 Antibody (M06618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (M06618-1).
VPS15/PIK3R4 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-VPS15/PIK3R4 Antibody (M06618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-PI 3 Kinase R4 Rabbit Monoclonal Antibody (M06618-1)
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