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Product Info Summary
| SKU: | A01721-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Piezo1 (extracellular domain) Antibody
SKU/Catalog Number
A01721-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Piezo1 (extracellular domain) Antibody catalog # A01721-1. Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-Piezo1 (extracellular domain) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01721-1)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human Piezo1 (extracellular domain) recombinant protein (Position: G70-S373).
Reactive Species
A01721-1 is reactive to PIEZO1 in Human, Mouse
Observed Molecular Weight
300 kDa
Background of PIEZO1
The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with dehydrated hereditary stomatocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01721-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
ELISA, 1:100-1000
Positive Control
WB: human SiHa whole cell, human MCF-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Piezo1 using anti-Piezo1 antibody (A01721-1).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Piezo1 antigen affinity purified polyclonal antibody (A01721-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Piezo1 at approximately 300 kDa. The expected band size for Piezo1 is at 287 kDa.
Specific Publications For Anti-Piezo1 (extracellular domain) Antibody (A01721-1)
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