Product Info Summary
SKU: | A04771-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PIKE AGAP2 Antibody
SKU/Catalog Number
A04771-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PIKE AGAP2 Antibody (Catalog # A04771-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
PIKE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PIKE AGAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04771-1)
Host
Rabbit
Contents
PIKE Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE. The immunogen is located within the last 50 amino acids of PIKE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.
Reactive Species
A04771-1 is reactive to Agap2 in Human, Mouse
Applications
A04771-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
124.438kDa
Background of Agap2
Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5
Click image to see more details
Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Agap2 (Source: Uniprot.org, NCBI)
Gene Name
Agap2
Full Name
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Weight
124.438kDa
Superfamily
centaurin gamma-like family
Alternative Names
AGAP-2; ArfGAP with GTPase domain, ankyrin repeat and PH domain 2; arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; centaurin, gamma 1; centaurin-gamma-1; CENTG1; cnt-g1; FLJ16430; GGAP2; GTP-binding and GTPase activating protein 2; GTP-binding and GTPase-activating protein 2; KIAA0167; Phosphatidylinositol-3-kinase enhancer; phosphoinositide 3-kinase enhancer; PIKEArf GAP with GTP-binding protein-like, ANK repeat and PH domains 2 Agap2|Centg1, Pike|ArfGAP with GTPase domain, ankyrin repeat and PH domain 2|arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2|AGAP-2|centaurin, gamma 1|cnt-g1|nuclear GTPase PIKE|phosphatidylinositol 3-kinase enhancer
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Agap2, check out the Agap2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Agap2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIKE AGAP2 Antibody (A04771-1)
Hello CJ!
No publications found for A04771-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PIKE AGAP2 Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-PIKE AGAP2 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question