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Product Info Summary
| SKU: | A00201 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PINK1 Antibody
SKU/Catalog Number
A00201
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PINK1 Antibody (Catalog # A00201). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PINK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-PINK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00201)
Host
Rabbit
Contents
PINK1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1. The immunogen is located within amino acids 120 - 170 of PINK1.
Cross-reactivity
PINK1 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will only detect the longer isoform. PINK1 antibody will detect the cleaved and uncleaved form of PINK1.
Reactive Species
A00201 is reactive to PINK1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
62.8 kDa
Background of PINK1
The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1). PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2). PINK1 is cleaved by the mitochondrial protease PARL (3). Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00201 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PINK1 in A431 cell lysate with PINK1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of PINK1 in mouse brain tissue with PINK1 Antibody at 5 μg/mL.
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Immunofluorescence of PINK1 in mouse brain tissue with PINK1 Antibody at 20 μg/mL.
Green: PINK1 antibody (A00201)
Red: Phylloidin staining
Blue: DAPI staining
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Western blot analysis of PINK1 in (A) mouse and (B) rat spleen tissue lysate with PINK1 antibody at 1 μg/mL.
Specific Publications For Anti-PINK1 Antibody (A00201)
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