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- Table of Contents
PINK1 antibody (A00201-2) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Flow Cytometry analysis of 293T cells using anti-PINK1 antibody (A00201-2).
Overlay histogram showing 293T cells stained with A00201-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PINK1 Antibody (A00201-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing 293T cells stained with A00201-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PINK1 Antibody (A00201-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for PINK1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PINK1 Western Blot GuideBundle Save
Anti-PINK1 Antibody Picoband®
PINK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
PINK1 antibody was positively detected by WB in:
human A431 whole cell, human HELA whole cell, human HEPG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PINK1 antibody was positively detected by ICC/IF in:
A431 cell
PINK1 antibody was positively detected by FC in:
293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-PINK1 Antibody Picoband®
View all PINK1 Antibodies
View all PINK1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PINK1 recombinant protein (Position: E112-A570).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
64 kDa
Calculated Molecular Weight
62.8 kDa
Background of PINK1
PTEN-induced kinase 1 (PINK1) is a mitochondrial serine/threonine-protein kinase encoded by the PINK1 gene. This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.
Cite This Product
Anti-PINK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00201-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
88%
Moderate Homology
Cow
86%
Moderate Homology
Sheep
85%
Moderate Homology
Rat
83%
Moderate Homology
Rabbit
83%
Moderate Homology
Mouse
82%
Moderate Homology
Zebrafish
58%
Low homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PINK1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PINK1 antibody
Flow Cytometry (FC) protocol for PINK1 antibody
Publications
Application:
Species:
EBV-Driven Rewiring of Drp1-Mediated Mitophagy Exacerbates Trichloroethylene-Induced Hypersensitivity Syndrome
Published: 2026-01-20
Journal: ENVIRONMENTAL POLLUTION
Application: WB,IF
Impact Factor: 7.30
Species: Human,Mouse
PMID: 41570879
Study on the molecular mechanism of KPNA2 regulation of myocardial ischemia/delayed reperfusion injury through mitophagy
Published: 2025-10-01
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 3.70
Species: Rat
PMID: 41043762
Gestational Exposure to Black Phosphorus Nanoparticles Induces Placental Trophoblast Dysfunction by Triggering Reactive Oxygen Species-Regulated Mitophagy
Published: 2025-04-23
Journal: ACS Nano
Application: WB
Impact Factor: 16.00
Species: Mouse
PMID: 40264356
Knockdown of NDUFAF6 inhibits breast cancer progression via promoting mitophagy and apoptosis
Published: 2024-12-20
Journal: CANCER BIOLOGY & THERAPY
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 39706687
Comparative analysis of primate and pig cells reveals primate-specific PINK1 expression and phosphorylation
Published: 2024-03-03
Journal: ZOOLOGICAL RESEARCH
Application: WB
Impact Factor: 4.00
Species: Pig
PMID: 38287905
Atmospheric fine particulate matter (PM2.5) induces pulmonary fibrosis by regulating different cell fates via autophagy
Published: 2024-03-02
Journal: SCIENCE OF THE TOTAL ENVIRONMENT
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 38438032
Study on the neuroprotective effect of Zhimu-Huangbo extract on mitochondrial dysfunction in HT22 cells induced by D-galactose by promoting mitochondrial autophagy
Published: 2023-08-09
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 37567426
Robust intervention for oxidative stress-induced injury in periodontitis via controllably released nanoparticles that regulate the ROS-PINK1-Parkin pathway.
Published: 2022-12-15
Journal: Frontiers in Bioengineering and Biotechnology
Application: IHC
Impact Factor: 6.06
Species: Rat
PMID: 36588945
Review
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1 Review For Anti-PINK1 Antibody Picoband®
PINK1 antibody (A00201-2) was validated by WB using human HepG2 cells, showing a clear target band with clean background and reliable performance.
| SKU | A00201-2 |
|---|---|
| Application | Western Blot |
| Sample | Human HepG2 cells |
| Sample Processing Description | Total proteins were extracted from normally cultured HepG2 cells. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | PINK1 Antibody Picoband® |
| Primary Incubation | 1:3000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | PINK1 antibody (A00201-2) was validated by WB using normally cultured human HepG2 cells. The results showed a clear target band with clean background, demonstrating good antibody performance. |
Lili Hu, Shanxi Medical University
Verified customer
Submitted 2026-07-07
Product Promise
We promise Anti-PINK1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00201-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026