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Product Info Summary
| SKU: | A10475 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PIPOX/Sarcosine Oxidase Antibody
View all Pipecolic acid oxidase Antibodies
SKU/Catalog Number
A10475
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PIPOX/Sarcosine Oxidase Antibody (Catalog # A10475). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIPOX/Sarcosine Oxidase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10475)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PIPOX.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10475 is reactive to Pipox in Human, Mouse
Applications
A10475 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43.847kDa
Background of Pipecolic acid oxidase
Metabolizes sarcosine, L-pipecolic acid and L-proline.
Ijlst L., Biochem. Biophys. Res. Commun. 270:1101-1105(2000).
Dodt G., Biochem. J. 345:487-494(2000).
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from NIH/3T3 cells and RAW264.7 cells, using PIPOX antibody A10475.
Protein Target Info & Infographic
Gene/Protein Information For Pipox (Source: Uniprot.org, NCBI)
Gene Name
Pipox
Full Name
Peroxisomal sarcosine oxidase
Weight
43.847kDa
Superfamily
MSOX/MTOX family
Alternative Names
EC 1.5.3.1; EC 1.5.3.7; L-pipecolate oxidase; L-pipecolic acid oxidase; LPIPOXperoxisomal sarcosine oxidase; pipecolic acid oxidase; PSO
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pipox, check out the Pipox Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pipox: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIPOX/Sarcosine Oxidase Antibody (A10475)
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No publications found for A10475
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-PIPOX/Sarcosine Oxidase Antibody
Question
We are currently using anti-PIPOX/Sarcosine Oxidase antibody A10475 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
P. Carter
Verified customer
Asked: 2019-10-17
Answer
The anti-PIPOX/Sarcosine Oxidase antibody (A10475) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-17
Question
Is a blocking peptide available for product anti-PIPOX/Sarcosine Oxidase antibody (A10475)?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We do provide the blocking peptide for product anti-PIPOX/Sarcosine Oxidase antibody (A10475). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-PIPOX/Sarcosine Oxidase antibody A10475 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-02
Question
Do you have a BSA free version of anti-PIPOX/Sarcosine Oxidase antibody A10475 available?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PIPOX/Sarcosine Oxidase antibody A10475 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-01
Question
Will anti-PIPOX/Sarcosine Oxidase antibody A10475 work on feline WB with liver?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
Our lab technicians have not validated anti-PIPOX/Sarcosine Oxidase antibody A10475 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PIPOX/Sarcosine Oxidase antibody A10475 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-21
Question
Please see the WB image, lot number and protocol we used for adrenal gland using anti-PIPOX/Sarcosine Oxidase antibody A10475. Please let me know if you require anything else.
W. Huang
Verified customer
Asked: 2015-01-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-15
Question
I have a question about product A10475, anti-PIPOX/Sarcosine Oxidase antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Jha
Verified customer
Asked: 2013-07-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10475 anti-PIPOX/Sarcosine Oxidase antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-10
