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Product Info Summary
| SKU: | M04066 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKN2/Prk2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04066
BM3971 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKN2/Prk2 Rabbit Monoclonal Antibody catalog # M04066. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKN2/Prk2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04066)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKN2
Reactive Species
M04066 is reactive to PKN2 in Human, Mouse, Rat
Observed Molecular Weight
112 kDa
Calculated molecular weight
112.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04066 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of PKN2 in 293T cell lysate.
Specific Publications For Anti-PKN2/Prk2 Rabbit Monoclonal Antibody (M04066)
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Customer Q&As
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5 Customer Q&As for Anti-PKN2/Prk2 Rabbit Monoclonal Antibody
Question
We are currently using anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066 for mouse tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
N. Collins
Verified customer
Asked: 2019-07-09
Answer
The anti-PKN2/Prk2 Rabbit Monoclonal antibody (M04066) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-09
Question
I see that the anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
G. Singh
Verified customer
Asked: 2019-05-29
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-29
Question
I have a question about product M04066, anti-PKN2/Prk2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04066 anti-PKN2/Prk2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-27
Question
Would anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066 work on bovine IP with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
Our lab technicians have not tested anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine leukemic t-cell in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-27
Question
Our lab want to know about to test anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066 on rat placenta spleen for research purposes, then I may be interested in using anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The products we sell, including anti-PKN2/Prk2 Rabbit Monoclonal antibody M04066, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-28
