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Product Info Summary
| SKU: | A10986 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PKR1 PROKR1 Antibody
SKU/Catalog Number
A10986
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PKR1 PROKR1 Antibody catalog # A10986. Tested in WB, IF, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKR1 PROKR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10986)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PKR1. AA range:19-68
Reactive Species
A10986 is reactive to PROKR1 in Human, Mouse, Rat
Calculated molecular weight
44.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10986 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IF/ICC1:100-500
ELISA 1:5000-20000
IHC 1:100-500
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using PKR1 Polyclonal Antibody
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Western blot analysis of lysates from COLO cells, using PKR1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-PKR1 PROKR1 Antibody (A10986)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PKR1 PROKR1 Antibody
Question
I am looking for to test anti-PKR1 antibody A10986 on rat testis for research purposes, then I may be interested in using anti-PKR1 antibody A10986 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The products we sell, including anti-PKR1 antibody A10986, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-10
Question
Will anti-PKR1 antibody A10986 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
According to the expression profile of testis, PROKR1 is highly expressed in testis. So, it is likely that anti-PKR1 antibody A10986 will work for WB with testis.
Boster Scientific Support
Answered: 2019-06-17
Question
Is there a BSA free version of anti-PKR1 antibody A10986 available?
A. Miller
Verified customer
Asked: 2018-05-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PKR1 antibody A10986 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-14
Question
We are currently using anti-PKR1 antibody A10986 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
E. Kulkarni
Verified customer
Asked: 2018-04-09
Answer
The anti-PKR1 antibody (A10986) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-09
Question
I see that the anti-PKR1 antibody A10986 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-01-24
Question
Would A10986 anti-PKR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Carter
Verified customer
Asked: 2017-02-22
Answer
As indicated on the product datasheet, A10986 anti-PKR1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-02-22
Question
Does anti-PKR1 antibody A10986 work on feline WB with adenohypophysis?
D. Jackson
Verified customer
Asked: 2017-02-13
Answer
Our lab technicians have not validated anti-PKR1 antibody A10986 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PKR1 antibody A10986 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline adenohypophysis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-13
