Click image to see more details
Product Info Summary
| SKU: | A05639 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Plakophilin 4 PKP4 Antibody
SKU/Catalog Number
A05639
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Plakophilin 4 PKP4 Antibody catalog # A05639. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plakophilin 4 PKP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05639)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PKP4. AA range:185-234
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05639 is reactive to PKP4 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
131.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05639 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Mouse Heart lysis using Plakophilin 4 antibody.
Specific Publications For Anti-Plakophilin 4 PKP4 Antibody (A05639)
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Customer Q&As
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5 Customer Q&As for Anti-Plakophilin 4 PKP4 Antibody
Question
Does A05639 anti-Plakophilin 4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
As indicated on the product datasheet, A05639 anti-Plakophilin 4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
Is a blocking peptide available for product anti-Plakophilin 4 antibody (A05639)?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
We do provide the blocking peptide for product anti-Plakophilin 4 antibody (A05639). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Plakophilin 4 antibody A05639. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-12
Question
We are currently using anti-Plakophilin 4 antibody A05639 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
The anti-Plakophilin 4 antibody (A05639) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-02
Question
Would anti-Plakophilin 4 antibody A05639 work for WB with cervix carcinoma erythroleukemia?
T. Krishna
Verified customer
Asked: 2015-04-15
Answer
According to the expression profile of cervix carcinoma erythroleukemia, PKP4 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Plakophilin 4 antibody A05639 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2015-04-15
