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- Table of Contents
PLBD2 antibody (A12971) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HepG2 cells using anti-PLBD2 antibody (A12971).
Overlay histogram showing HepG2 cells stained with A12971 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLBD2 Antibody (A12971, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing HepG2 cells stained with A12971 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLBD2 Antibody (A12971, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-PLBD2 Antibody Picoband®
PLBD2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
PLBD2 antibody was positively detected by WB in:
human HepG2 whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PLBD2 antibody was positively detected by IHC in:
human lung adenocarcinoma tissue, human prostate adenocarcinoma tissue, human rectum tissue, human rectum tissue, human spleen tissue, human spleen tissue, human testicular seminoma tissue, human testicular seminoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PLBD2 antibody was positively detected by ICC/IF in:
U2OS cell
PLBD2 antibody was positively detected by IF in:
human lung cancer tissue
PLBD2 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg /1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PLBD2 Antibody Picoband®
View all PLBD2 Antibodies
View all PLBD2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PLBD2 recombinant protein (Position: Q108-Q525). Human PLBD2 shares 88% and 87.1% amino acid (aa) sequence identity with mouse and rat PLBD2, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
75 kDa
Calculated Molecular Weight
65.5 kDa
Background of PLBD2
Putative Phospholipase B-like (PLBD2) is a 585 amino acids lipoprotein lipase (LPL) encoded by the or Plbd2 gene. PLBD2 is considered a difficult-to-remove Host Cell Protein (HCP) from Chinese hamster ovary (CHO) cells derived therapeutic antibodies. Some HCPs could cause immunogenic responses in patients if not removed.
Cite This Product
Anti-PLBD2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12971)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Mouse
88%
Moderate Homology
Rat
87%
Moderate Homology
Dog
87%
Moderate Homology
Sheep
86%
Moderate Homology
Cow
86%
Moderate Homology
Rabbit
85%
Moderate Homology
Zebrafish
66%
Low homology
Drosophila
32%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PLBD2 antibody
Immunohistochemistry (IHC) protocol for PLBD2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PLBD2 antibody
Immunofluorescence (IF) protocol for PLBD2 antibody
Flow Cytometry (FC) protocol for PLBD2 antibody
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Product Promise
We promise Anti-PLBD2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A12971
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026