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Product Info Summary
| SKU: | M00677 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00677
BM4477 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody catalog # M00677. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00677)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FAA-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PLCG1
Reactive Species
M00677 is reactive to PLCG1 in Human, Mouse, Rat
Observed Molecular Weight
149 kDa
Calculated molecular weight
148.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00677 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human Jurkat whole cell, human MCF-7 whole cell, human HepG2 whole cell, human SH-SY5Y whole cell, rat C6 whole cell, rat RH35 whole cell
Validation Images & Assay Conditions
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Western blot analysis of PLCG1 using anti-PLCG1 antibody (M00677).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat RH35 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCG1 antigen affinity purified monoclonal antibody (Catalog # M00677) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCG1 at approximately 149 kDa. The expected band size for PLCG1 is at 149 kDa.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody (M00677)
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Customer Q&As
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6 Customer Q&As for Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody
Question
We are currently using anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody (M00677) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-07
Question
I am interested in to test anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677 on human thymocyte for research purposes, then I may be interested in using anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The products we sell, including anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-02
Question
Is a blocking peptide available for product anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody (M00677)?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We do provide the blocking peptide for product anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody (M00677). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-04
Question
Would M00677 anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can see on the product datasheet, M00677 anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-03
Question
Can you help my question with product M00677, anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Anderson
Verified customer
Asked: 2018-09-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00677 anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymocyte using anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-16
