Anti-Phospholipase D4 PLD4 Antibody

PLD4/Phospholipase D4 antibody

Boster Bio Anti-Phospholipase D4 PLD4 Antibody (Catalog # A13908). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A13908
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Phospholipase D4 PLD4 Antibody

View all PLD4/Phospholipase D4 Antibodies

SKU/Catalog Number

A13908

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospholipase D4 PLD4 Antibody (Catalog # A13908). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospholipase D4 PLD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13908)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human PLD4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A13908 is reactive to Pld4 in Human, Mouse, Rat

Applications

A13908 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

56.154kDa

Background of PLD4/Phospholipase D4

Belongs to the phospholipase D family.

Clark H.F., Genome Res. 13:2265-2270(2003).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Tao W.A., Nat. Methods 2:591-598(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Pld4 (Source: Uniprot.org, NCBI)

Gene Name

Pld4

Full Name

5'-3' exonuclease PLD4

Weight

56.154kDa

Superfamily

phospholipase D family

Alternative Names

C14orf175; Choline phosphatase 4; chromosome 14 open reading frame 175; EC 3.1.4.4; Phosphatidylcholine-hydrolyzing phospholipase D4; phospholipase D family, member 4; phospholipase D4; PLD 4 Pld4|AI132321, th, thss|phospholipase D family, member 4|5-3 exonuclease PLD4|PLD 4|choline phosphatase 4|phosphatidylcholine-hydrolyzing phospholipase D4|phospholipase D4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Pld4, check out the Pld4 Infographic

Pld4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Pld4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A13908 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism

Shan S,Wu C,Shi J,Zhang X,Niu J,Li H,Li Z.Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.J Agric Food Chem.2020 Aug 5;68(31):8295-8307.doi:10.1021/acs.jafc.0c03257.Epub 2020 Jul 27.PMID:32657580.
Species: Human,Mouse
A13908 usage in article: APP:WB, SAMPLE:HCT-116 CELL AND DLD1 CELL, DILUTION:NA

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7 Customer Q&As for Anti-Phospholipase D4 PLD4 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-PLD4 antibody A13908. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-20

Question

We are currently using anti-PLD4 antibody A13908 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

The anti-PLD4 antibody (A13908) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-06

Question

I see that the anti-PLD4 antibody A13908 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-05

Question

I was wanting to use your anti-PLD4 antibody for WB for human b-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human b-cell identification?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

It shows on the product datasheet, A13908 anti-PLD4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-13

Question

Here is the WB image, lot number and protocol we used for b-cell using anti-PLD4 antibody A13908. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-03-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-25

Question

Will A13908 anti-PLD4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

It shows on the product datasheet, A13908 anti-PLD4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-22

Question

Does anti-PLD4 antibody A13908 work for WB with b-cell?

S. Miller

Verified customer

Asked: 2017-08-14

Answer

According to the expression profile of b-cell, PLD4 is highly expressed in b-cell. So, it is likely that anti-PLD4 antibody A13908 will work for WB with b-cell.

Boster Scientific Support

Answered: 2017-08-14

Order DetailsPrice
A13908-100uL

100uL

$360

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