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Product Info Summary
| SKU: | A02150-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Trem2 Antibody Picoband®
SKU/Catalog Number
A02150-3
Size
100 μg/vial
Form
Lyophilized
Description
TREM2 is a myeloid-lineage receptor that signals via adaptor proteins (e.g., TYROBP/DAP12) and is widely studied in microglial activation, phagocytosis, and neuroinflammatory responses (disease context: neurodegeneration/Alzheimer’s biology, putative). Assay context: validated for ELISA, Flow Cytometry, and WB; when comparing myeloid phenotypes, it is often interpreted alongside macrophage markers such as CD163 and innate sensors like TLR2 (fluorophore labeling for flow readouts can be approached via antibody conjugation when needed).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Trem2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02150-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Trem2 recombinant protein (Position: L19-D131).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02150-3 is reactive to Trem2 in Rat
Observed Molecular Weight
28 kDa
Background of Trem2
Triggering receptor expressed on myeloid cells 2 also known as TREM-2 is a protein that in humans is encoded by the TREM2 gene. This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02150-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Rat
ELISA, 0.1-0.5μg/ml,
Positive Control
WB: rat C6 whole cell
FCM: RH35 cell
Validation Images & Assay Conditions
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Western blot analysis of Trem2 using anti-Trem2 antibody (A02150-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Trem2 antigen affinity purified polyclonal antibody (Catalog # A02150-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Trem2 at approximately 28KD. The expected band size for Trem2 is at 28KD.
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Flow Cytometry analysis of RH35 cells using anti-Trem2 antibody (A02150-3).
Overlay histogram showing RH35 cells stained with A02150-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Trem2 Antibody (A02150-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Trem2 Antibody Picoband® (A02150-3)
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