Anti-PLEKHM3 Antibody

Product Name

Anti-PLEKHM3 Antibody

View all PLEKHM3 Antibodies

SKU/Catalog Number

A16310

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PLEKHM3 Antibody (Catalog # A16310). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PLEKHM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PLEKHM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16310)

Host

Rabbit

Contents

PLEKHM3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PLEKHM3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM3. The immunogen is located within the last 50 amino acids of PLEKHM3.

Reactive Species

A16310 is reactive to PLEKHM3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

87.2 kDa

Background of PLEKHM3

PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A16310 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PLEKHM3

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