Anti-PLET1 Antibody Picoband™

Product Name

Anti-PLET1 Antibody Picoband™

View all PLET-1 Antibodies

SKU/Catalog Number

A14923-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PLET1 Antibody Picoband™ catalog # A14923-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PLET1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14923-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PLET1 recombinant protein (Position: D46-K166).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A14923-1 is reactive to Plet1 in Human

Applications

A14923-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

25.006kDa

Background of PLET-1

PLET-1 (placenta -expressed transcript 1), also known as antigen mAgK114, is a 23 kDa membrane protein that is probably GPI-anchored. It is recognized by the antibodies MTS20 and MTS24. Mature mouse PLET-1, which most likely includes amino acids (aa) 28-216 of 237 aa, shares less than 60% aa sequence identity with the most closely related rat or human protein. A potential 194 aa mouse splice variant diverges after aa 149 but still contains a hydrophobic sequence at the Cterminus. PLET-1 is a marker of early thymic progenitor cells and pancreatic duct epithelium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg /1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PLET1 using anti-PLET1 antibody (A14923-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT-1080 whole cell lysates,
Lane 2: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLET1 antigen affinity purified polyclonal antibody (Catalog # A14923-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLET1 at approximately 28 kDa. The expected band size for PLET1 is at 23,25 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of SiHa cells using anti-PLET1 antibody (A14923-1).
Overlay histogram showing SiHa cells stained with A14923-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLET1 Antibody (A14923-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Plet1 (Source: Uniprot.org, NCBI)

Alternative Names

AgK114; C11orf34; chromosome 11 open reading frame 34; placenta expressed transcript 1; PLET1; PLET-1 Plet1|0610037B23Rik, 1600029D21Rik, AA407923, AU043048, PLE|placenta expressed transcript 1|placenta-expressed transcript 1 protein|antigen mAgK114

For more info on Plet1, check out the Plet1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Plet1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Plet1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis