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- Table of Contents
PLIN3 antibody (A02977-1) performs well in ELISA, Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of TIP47/PLIN3 using anti-TIP47/PLIN3 antibody (A02977-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIP47/PLIN3 antigen affinity purified polyclonal antibody (A02977-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIP47/PLIN3 at approximately 47 kDa. The expected band size for TIP47/PLIN3 is at 47 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIP47/PLIN3 antigen affinity purified polyclonal antibody (A02977-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIP47/PLIN3 at approximately 47 kDa. The expected band size for TIP47/PLIN3 is at 47 kDa.
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Anti-TIP47/PLIN3 Antibody
PLIN3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IP, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunocytochemistry/Immunofluorescence
1:50-400
Immunoprecipitation
1:50
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
1:100-1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-TIP47/PLIN3 Antibody
View all PLIN3 Antibodies
View all PLIN3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human TIP47/PLIN3 recombinant protein (Position: M135-P430).
Calculated Molecular Weight
47.1 kDa
Background of PLIN3
Mannose-6-phosphate receptor binding protein 1 (M6PRBP1), also known as Perilipin 3 (PLN3) or TIP47, is a protein which in humans is encoded by the M6PRBP1 gene. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-TIP47/PLIN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02977-1)
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Product Promise
We promise Anti-TIP47/PLIN3 Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02977-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026