Anti-PNN/Pinin Antibody

Pinin antibody

Boster Bio Anti-PNN/Pinin Antibody (Catalog # A01590). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A01590
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-PNN/Pinin Antibody

View all Pinin Antibodies

SKU/Catalog Number

A01590

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PNN/Pinin Antibody (Catalog # A01590). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PNN/Pinin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01590)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human PNN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01590 is reactive to PNN in Human, Mouse

Applications

A01590 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

81.628kDa

Background of Pinin

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.

Ouyang P., J. Cell Biol. 135:1027-1042(1996).
Degen W.G.J., Biochim. Biophys. Acta 1444-384-394(1999).
Shi Y., Oncogene 19:289-297(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For PNN (Source: Uniprot.org, NCBI)

Gene Name

PNN

Full Name

Pinin

Weight

81.628kDa

Superfamily

pinin family

Alternative Names

140 kDa nuclear and cell adhesion-related phosphoprotein; Desmosome-associated protein; Domain-rich serine protein; DRS protein; DRSP; DRSSR-like protein; Melanoma metastasis clone A protein; memA; neutrophil protein; Nuclear protein SDK3; pinin; pinin, desmosome associated protein; SDK3 PNN DRS, DRSP, SDK3, memA pinin, desmosome associated protein pinin|140 kDa nuclear and cell adhesion-related phosphoprotein|SR-like protein|desmosome-associated protein|domain-rich serine protein|melanoma metastasis clone A protein|neutrophil protein|nuclear protein SDK3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PNN, check out the PNN Infographic

PNN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PNN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-PNN/Pinin Antibody

Question

Is a blocking peptide available for product anti-PNN/Pinin antibody (A01590)?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

We do provide the blocking peptide for product anti-PNN/Pinin antibody (A01590). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-10

Question

We are currently using anti-PNN/Pinin antibody A01590 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?

P. Li

Verified customer

Asked: 2019-07-03

Answer

The anti-PNN/Pinin antibody (A01590) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-03

Question

Do you have a BSA free version of anti-PNN/Pinin antibody A01590 available?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PNN/Pinin antibody A01590 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-02-11

Question

I have attached the WB image, lot number and protocol we used for keratinocyte using anti-PNN/Pinin antibody A01590. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-24

Order DetailsPrice
A01590-100uL

100uL

$360

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