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Product Info Summary
| SKU: | A09557-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PNPLA1 Antibody Picoband®
SKU/Catalog Number
A09557-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PNPLA1 Antibody Picoband® catalog # A09557-1. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PNPLA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09557-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PNPLA1 recombinant protein (Position: K141-D496). Human PNPLA1 shares 51.5% amino acid (aa) sequence identity with mouse PNPLA1.
Reactive Species
A09557-1 is reactive to PNPLA1 in Human
Observed Molecular Weight
58 kDa
Calculated molecular weight
57.9 kDa
Background of PNPLA1
PNPLA1 is important in the formation of the epidermal lipid barrier and plays a role in glycerophospholipid metabolism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09557-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of PNPLA1 using anti-PNPLA1 antibody (A09557-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPLA1 antigen affinity purified polyclonal antibody (Catalog # A09557-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPLA1 at approximately 58 kDa. The expected band size for PNPLA1 is at 58 kDa.
Specific Publications For Anti-PNPLA1 Antibody Picoband® (A09557-1)
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