Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody

Product Name

Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody

View all Podoplanin Antibodies

SKU/Catalog Number

M01124

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody (Catalog # M01124). Tested in ELISA, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01124)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PDPN/1433

Isotype

IgG1, kappa

Immunogen

Recombinant human Podoplanin (PDPN) protein fragment (around aa 24-126) (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01124 is reactive to PDPN in Human

Applications

M01124 is guaranteed for ELISA, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

16.698kDa

Background of Podoplanin

This monoclonal antibody recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating, order antibody without BSA)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Cervix stained with Anti-Podoplanin-Monospecific Mouse Monoclonal Antibody (PDPN/1433)

Gene/Protein Information For PDPN (Source: Uniprot.org, NCBI)

Alternative Names

36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha PDPN AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, T1A2, TI1A podoplanin podoplanin|PA2.26 antigen|T1-alpha|glycoprotein 36|glycoprotein, 36-KD|hT1alpha-1|hT1alpha-2|lung type I cell membrane associated glycoprotein|lung type-I cell membrane-associated glycoprotein (T1A-2)

For more info on PDPN, check out the PDPN Infographic

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In this infographic, you will see the following information for PDPN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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