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- Table of Contents
Pdpn antibody (A01124-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of RH35 cells using anti-Podoplanin/gp36/Pdpn antibody (A01124-2).
Overlay histogram showing RH35 cells stained with A01124-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Podoplanin/gp36/Pdpn Antibody (A01124-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RH35 cells stained with A01124-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Podoplanin/gp36/Pdpn Antibody (A01124-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Podoplanin/gp36/Pdpn Antibody Picoband®
Pdpn antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
Pdpn antibody was positively detected by WB in:
rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Pdpn antibody was positively detected by IHC in:
mouse lung tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Pdpn antibody was positively detected by ICC/IF in:
HEPA1-6 cell
Pdpn antibody was positively detected by FC in:
RH35 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Rat
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Podoplanin/gp36/Pdpn Antibody Picoband®
View all PDPN Antibodies
View all PDPN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Podoplanin/gp36/Pdpn recombinant protein (Position: T24-P172).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated Molecular Weight
18.2 kDa
Background of Pdpn
Podoplanin (PDPN) is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The International radiation Hybrid mapping consortium mapped the T1A gene to chromosome 1. It has got 162 amino acid protein which is a typical type I membrane glycoprotein, with an N-terminal signal sequence, a signal sequence cleavage site, a C-terminal hydrophobic stretch, and a short cytoplasmic tail. Northern blot analysis detected a 1.2-kb T1A2 transcript only in lung. It has been described as a differentiation antigen and influenza-virus receptor, and a marker of lung injury.
Cite This Product
Anti-Podoplanin/gp36/Pdpn Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01124-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
77%
Moderate Homology
Dog
53%
Low homology
Rabbit
46%
Low homology
Human
45%
Low homology
Monkey
45%
Low homology
Cow
39%
Low homology
Sheep
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Pdpn antibody
Immunohistochemistry (IHC) protocol for Pdpn antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Pdpn antibody
Flow Cytometry (FC) protocol for Pdpn antibody
Publications
Application:
Species:
Gestational PFOS Exposure Induces Alveolar Developmental Defects by Affecting Immune–Epithelial Crosstalk in Offspring Rats
Published: 2026-06-14
Journal: JOURNAL OF APPLIED TOXICOLOGY
Application: IF
Impact Factor: 3.10
Species: Rat
PMID: 42289312
Esaxerenone Attenuates Aldosterone-Induced Renal Fibrosis by Suppressing Fibroblast-to-Lymphatic Endothelial-like Cell Transdifferentiation
Published: 2026-05-12
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB,IHC
Impact Factor: 5.60
Species: Rat
PMID: 42196278
Identification of Lymphangiogenesis-Related Diagnostic Model for Predicting Abdominal Aortic Aneurysm Onset and Progression and Validation of Lymphopoiesis in Abdominal Aortic Aneurysm
Published: 2025-11-01
Journal: MOLECULAR AND CELLULAR PROBES
Application: WB,IHC
Impact Factor: 3.00
Species: Mouse
PMID: 41183720
Podoplanin-TGF-β1 Autocrine Loop: Pivotal Regulator of Islet Stellate Cell Activation via Cell Deformation, Orchestrating Islet Fibrosis in Diabetes
Published: 2025-09-03
Journal: FASEB JOURNAL
Application: IF
Impact Factor: 4.20
Species: Mouse
PMID: 40899778
Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression
Published: 2023-02-03
Journal: Molecular and Clinical Oncology
Application: IHC
Impact Factor: 1.20
Species: Human
PMID: 36844464
Lymphatic endothelial cell-secreted CXCL1 stimulates lymphangiogenesis and metastasis of gastric cancer
Published: 2011-03-08
Journal: INTERNATIONAL JOURNAL OF CANCER
Application: ICC
Impact Factor: 4.93
Species: Rat
PMID: 21387301
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Product Promise
We promise Anti-Podoplanin/gp36/Pdpn Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A01124-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026