Product Info Summary
SKU: | A11270 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-POLR2H Antibody
SKU/Catalog Number
A11270
Size
100ul
Form
Liquid
Description
Boster Bio Anti-POLR2H Antibody catalog # A11270. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLR2H Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11270)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ATP5G2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11270 is reactive to POLR2H in Human, Mouse, Rat
Applications
A11270 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
17.143kDa
Background of RPB8
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. HAMAP-Rule MF_01396
Dyer M.R., Biochem. J. 293:51-64(1993).
Higuti T., Biochim. Biophys. Acta 1173:87-90(1993).
Farrell L.B., Biochem. Biophys. Res. Commun. 144:1257-1264(1987).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-POLR2H Antibody A11270
Western blot (WB) analysis of POLR2H polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat iver tissue lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For POLR2H (Source: Uniprot.org, NCBI)
Gene Name
POLR2H
Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Weight
17.143kDa
Superfamily
eukaryotic RPB8 RNA polymerase subunit family
Alternative Names
DNA-directed RNA polymerases I, II, and III subunit RPABC3; hsRPB8; II, and III 17.1 kDa polypeptide; II, and III subunit ABC3; polymerase (RNA) II (DNA directed) polypeptide H; RPB8 homolog POLR2H RPABC3, RPB17, RPB8 RNA polymerase II, I and III subunit H DNA-directed RNA polymerases I, II, and III subunit RPABC3|DNA-directed RNA polymerase II subunit H|DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide|RNA polymerase II subunit H|RPB8 homolog|polymerase (RNA) II (DNA directed) polypeptide H|polymerase (RNA) II subunit H
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2H, check out the POLR2H Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2H: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR2H Antibody (A11270)
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No publications found for A11270
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