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Product Info Summary
| SKU: | A18320 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-POLR2J3 Antibody (N-term)
SKU/Catalog Number
A18320
Size
80 µl
Description
Boster Bio Anti-POLR2J3 Antibody (N-term) (Catalog # A18320). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-POLR2J3 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18320)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This POLR2J3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-25 amino acids from the N-terminal region of human POLR2J3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A18320 is reactive to POLR2J3 in Human
Reconstitution
Calculated molecular weight
13092 Da
Background of POLR2J3
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18320 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot analysis of lysate from MCF-7 cell line,using POLR2J3 Antibody (N-term). A18320 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Protein Target Info & Infographic
Gene/Protein Information For POLR2J3 (Source: Uniprot.org, NCBI)
Gene Name
POLR2J3
Full Name
DNA-directed RNA polymerase II subunit RPB11-b2
Weight
13092 Da
Superfamily
archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family
Alternative Names
DNA-directed RNA polymerase II subunit RPB11-b2, RNA polymerase II subunit B11-b2, RPB11b2, DNA-directed RNA polymerase II subunit J3, POLR2J3 POLR2J3 POLR2J2, RPB11b1, RPB11b2 RNA polymerase II subunit J3 DNA-directed RNA polymerase II subunit RPB11-b2|DNA-directed RNA polymerase II subunit 11|DNA-directed RNA polymerase II subunit J2|DNA-directed RNA polymerase II subunit J3|DNA-directed RNA polymerase II subunit RPB11-b1|RNA polymerase II subunit B11-b1|RNA polymerase II subunit B11-b2|polymerase (RNA) II (DNA directed) polypeptide J3|polymerase (RNA) II subunit J3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2J3, check out the POLR2J3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2J3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR2J3 Antibody (N-term) (A18320)
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