Anti-PPAR alpha Antibody

Product Name

Anti-PPAR alpha Antibody

View all PPAR alpha/NR1C1 Antibodies

SKU/Catalog Number

A00600

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PPAR alpha Antibody (Catalog # A00600). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-PPAR alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00600)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00600 is reactive to PPARA in Human, Mouse

Applications

A00600 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

52.225kDa

Background of PPAR alpha/NR1C1

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements. Anti-Ppar Antibody is useful for research interested in transcription and metabolic pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:75,000 - 1:125,000
Flow Cytometry: User optimized
IHC: 1:100-1:300
IF Microscopy: 1-5µg/mL
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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PPAR alpha was detected in paraffin-embedded sections of rat brain tissues using rabbit anti-PPAR alpha (N-terminal specific) affinity purified polyclonal antibody (Catalog # A00600) at 1:200. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones)

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PPAR alpha was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti-PPAR alpha (N-terminal specific) affinity purified polyclonal antibody (Catalog # A00600) at 1:50. Images show nuclear staining in hepatocytes (10 and 40x microscope magnification). The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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ELISA results of rabbit anti-PPAR alpha (N-terminal specific) affinity purified polyclonal antibody (Catalog # A00600) tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1

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Western blot analysis of PPAR alpha expression in HEK293 cell lysates (lane 1), HeLa cell lysates (lane 2), MCF-7 cell lysates (lane 3), Jurkat cell lysates (lane 4), A431 cell lysates (lane 5), LNCaP cell lysates (lane 6), A-172 cell lysates (lane 7) and NIH/3T3 cell lysates (lane 8). Load: 35

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Western blot analysis of PPAR alpha expression in 10

Gene/Protein Information For PPARA (Source: Uniprot.org, NCBI)

Alternative Names

alpha; hPPAR; NR1C1; NR1C1MGC2237; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferator-activated receptor alpha; PPAR alpha; PPARA; PPARalpha; PPAR-alpha; PPARMGC2452 PPARA NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator activated receptor alpha peroxisome proliferator-activated receptor alpha|PPAR-alpha|nuclear receptor subfamily 1 group C member 1|peroxisome proliferative activated receptor, alpha|peroxisome proliferator-activated nuclear receptor alpha variant 3

For more info on PPARA, check out the PPARA Infographic

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In this infographic, you will see the following information for PPARA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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