Anti-PPEF1 Antibody Picoband™

Product Name

Anti-PPEF1 Antibody Picoband™

View all PPEF1 Antibodies

SKU/Catalog Number

A11074-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPEF1 Antibody Picoband™ catalog # A11074-1. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PPEF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11074-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human PPEF1 recombinant protein (Position: H137-L652). Human PPEF1 shares 72.7% and 73.2% amino acid (aa) sequence identity with mouse and rat PPEF1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11074-1 is reactive to PPEF1 in Human

Applications

A11074-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

76 kDa

Calculated molecular weight

75.792kDa

Background of PPEF1

Serine/threonine-protein phosphatase with EF-hands 1 is an enzyme that in humans is encoded by the PPEF1 gene. This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PPEF1 using anti-PPEF1 antibody (A11074-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPEF1 antigen affinity purified polyclonal antibody (Catalog # A11074-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPEF1 at approximately 76 kDa. The expected band size for PPEF1 is at 76 kDa.

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Figure 2. IF analysis of PPEF1 using anti-PPEF1 antibody (A11074-1).
PPEF1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPEF1 Antibody (A11074-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of 293T cells using anti-PPEF1 antibody (A11074-1).
Overlay histogram showing 293T cells stained with A11074-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPEF1 Antibody (A11074-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PPEF1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.3.16; PP7; PPEF; PPEF-1; PPP7C; PPP7CA; protein phosphatase 7, catalytic subunit, alpha isozyme; Protein phosphatase with EF calcium-binding domain; protein phosphatase, EF-hand calcium binding domain 1; protein phosphatase, serine/threonine type, with EF-hands; serine/threonine protein phosphatase 7; Serine/threonine-protein phosphatase 7; serine/threonine-protein phosphatase with EF-hands 1 PPEF1 PP7, PPEF, PPP7C, PPP7CA protein phosphatase with EF-hand domain 1 serine/threonine-protein phosphatase with EF-hands 1|protein phosphatase 7, catalytic subunit, alpha isozyme|protein phosphatase with EF calcium-binding domain|protein phosphatase, EF-hand calcium binding domain 1|protein phosphatase, serine/threonine type, with EF-hands|serine/threonine protein phosphatase 7

For more info on PPEF1, check out the PPEF1 Infographic

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In this infographic, you will see the following information for PPEF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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