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Product Info Summary
| SKU: | A09894-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-PPP2R5B Antibody (C-term)
SKU/Catalog Number
A09894-1
Size
80 µl
Description
Boster Bio Anti-PPP2R5B Antibody (C-term) (Catalog # A09894-1). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PPP2R5B Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09894-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PPP2R5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 466-500 amino acids from the C-terminal region of human PPP2R5B.
Reactive Species
A09894-1 is reactive to PPP2R5B in Human
Calculated molecular weight
57.4 kDa
Background of PPP2R5B
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09894-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent image of SH-SY5Y cells stained with PPP2R5B Antibody (C-term). A09894-1 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysate from SK-N-MC cell line, using PPP2R5B Antibody (C-term) (Cat. #A09894-1). A09894-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Specific Publications For Anti-PPP2R5B Antibody (C-term) (A09894-1)
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