Anti-PRAM1 Antibody Picoband™

Product Name

Anti-PRAM1 Antibody Picoband™

View all PRAM1 Antibodies

SKU/Catalog Number

A10037-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PRAM1 Antibody Picoband™ catalog # A10037-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PRAM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10037-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PRAM1 recombinant protein (Position: E361-R670).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10037-2 is reactive to PRAM1 in Human

Applications

A10037-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

73.969kDa

Background of PRAM1

PML-RARA-regulated adapter molecule 1 is a protein that in humans is encoded by the PRAM1 gene. The protein encoded by this gene is similar to FYN binding protein (FYB/SLAP-130), an adaptor protein involved in T cell receptor mediated signaling. This gene is expressed and regulated during normal myelopoiesis. The expression of this gene is induced by retinoic acid and is inhibited by the expression of PML-RARalpha, a fusion protein of promyelocytic leukemia (PML) and the retinoic acid receptor-alpha (RARalpha).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PRAM1 using anti-PRAM1 antibody (A10037-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAM1 antigen affinity purified polyclonal antibody (Catalog # A10037-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAM1 at approximately 90 kDa. The expected band size for PRAM1 is at 70,74,90-100 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-PRAM1 antibody (A10037-2).
Overlay histogram showing JK cells stained with A10037-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRAM1 Antibody (A10037-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PRAM1 (Source: Uniprot.org, NCBI)

Alternative Names

MGC39864; PML-RAR; PML-RARA regulated adaptor molecule 1; PML-RARA target gene encoding an Adaptor Molecule-1; PML-RARA-regulated adapter molecule 1; PRAM; PRAM-1 PRAM1 PML-RAR, PRAM-1 PML-RARA regulated adaptor molecule 1 PML-RARA-regulated adapter molecule 1|PML-RARA target gene encoding an Adaptor Molecule-1|PRAM

For more info on PRAM1, check out the PRAM1 Infographic

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In this infographic, you will see the following information for PRAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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