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- Table of Contents
PRDM11 antibody (A11092-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of THP-1 cells using anti-PRDM11 antibody (A11092-2).
Overlay histogram showing THP-1 cells stained with A11092-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM11 Antibody (A11092-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A11092-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM11 Antibody (A11092-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PRDM11 Antibody Picoband®
PRDM11 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
PRDM11 antibody was positively detected by WB in:
human U251 whole cell, human THP-1 whole cell, human Hela whole cell, human A549 whole cell, rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PRDM11 antibody was positively detected by IHC in:
human breast cancer tissue, human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PRDM11 antibody was positively detected by ICC/IF in:
Hela cell
PRDM11 antibody was positively detected by IF in:
human breast cancer tissue, human lung cancer tissue
PRDM11 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PRDM11 Antibody Picoband®
View all PRDM11 Antibodies
View all PRDM11 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PRDM11 recombinant protein (Position: E37-L483). Human PRDM11 shares 89.7% amino acid (aa) sequence identity with mouse PRDM11.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
58 kDa
Calculated Molecular Weight
57.9 kDa
Background of PRDM11
PRDM11 (PR/SET Domain 11) is a Protein Coding gene. Diseases associated with PRDM11 include Hermansky-Pudlak Syndrome 1. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and methyltransferase activity. An important paralog of this gene is PRDM7.
Cite This Product
Anti-PRDM11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11092-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
94%
High Homology
Cow
92%
High Homology
Sheep
91%
High Homology
Mouse
90%
High Homology
Rat
89%
Moderate Homology
Rabbit
87%
Moderate Homology
Zebrafish
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PRDM11 antibody
Immunohistochemistry (IHC) protocol for PRDM11 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PRDM11 antibody
Immunofluorescence (IF) protocol for PRDM11 antibody
Flow Cytometry (FC) protocol for PRDM11 antibody
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Product Promise
We promise Anti-PRDM11 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A11092-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026