Anti-PREX2 Antibody Picoband™

Product Name

Anti-PREX2 Antibody Picoband™

View all PREX2 Antibodies

SKU/Catalog Number

A06715-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PREX2 Antibody Picoband™ catalog # A06715-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PREX2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06715-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PREX2 recombinant protein (Position: D582-Q1094).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06715-1 is reactive to PREX2 in Human, Mouse

Applications

A06715-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

183 kDa

Calculated molecular weight

182.622kDa

Background of PREX2

Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 is a protein that in humans is encoded by the PREX2 gene. The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PREX2 using anti-PREX2 antibody (A06715-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse skeletal muscle lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX2 antigen affinity purified polyclonal antibody (Catalog # A06715-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX2 at approximately 183 kDa. The expected band size for PREX2 is at 183 kDa.

Gene/Protein Information For PREX2 (Source: Uniprot.org, NCBI)

Alternative Names

DEP domain-containing protein 2; DEP.2; DEPDC2; phosphatidylinositol 34,5-trisphosphate-dependent Rac exchanger 2 protein; phosphatidylinositol-34,5-trisphosphate-dependent Rac exchange factor 2; P-REX2,6230420N16Rik; PtdIns(34,5)-dependent Rac exchanger 2 PREX2 DEP.2, DEPDC2, P-REX2, PPP1R129 phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2 phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein|DEP domain-containing protein 2|PtdIns(3,4,5)-dependent Rac exchanger 2|protein phosphatase 1, regulatory subunit 129

For more info on PREX2, check out the PREX2 Infographic

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In this infographic, you will see the following information for PREX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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